[ PROPERTIES ] [ 属性 ]

Source: 源：  源：Prokaryotic expression 原核表达

Host: 主机：  主机：E.coli 大肠杆菌

Residues: 残留：  残留：Phe1700~His2115

Tags: 标签：  标签：N-terminal His Tag N-terminal His 标签

Subcellular Location: 亚细胞位置： Nucleus, Cytoplasm, Chromosome
细胞核、细胞质、染色体

Purity: 纯度：  纯度：> 95%

Traits: 性状：  性状：Freeze-dried powder 冻干粉

Buffer formulation: 缓冲液配方： PBS, pH7.4, containing 0.01% SKL, 5% Trehalose .
PBS，pH7.4，含0.01%SKL，5%海藻糖。

Original Concentration: 原始浓度：  原始浓度：350µg/mL 350微克/毫升

Applications: 应用： Positive Control; Immunogen; SDS-PAGE; WB.
阳性对照;免疫原;SDS-页面;工 务 局。

(May be suitable for use in other assays to be determined by the end user.)
（可能适用于最终用户确定的其他检测。

Predicted isoelectric point:
预测等电点： 
预测等电点：10.2

Predicted Molecular Mass:
预测分子量： 
预测分子量：48.7kDa

Accurate Molecular Mass: 精确分子量： 
准确的分子量：50/56kDa as determined by SDS-PAGE reducing conditions.
50/56kDa，由SDS-PAGE还原条件确定。

[ USAGE ] [ 用法 ]

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
在 10mM PBS （pH7.4） 中复溶至浓度为 0.1-1.0 mg/mL。不要涡旋。

[ STORAGE AND STABILITY ]
[ 储存及稳定性 ]

Storage: Avoid repeated freeze/thaw cycles.
储存：避免反复冻融循环。

 Store at 2-8ºC for one month.
在 2-8ºC 下储存一个月。

 Aliquot and store at -80ºC for 12 months.
分装并在 -80ºC 下储存 12 个月。

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined
稳定性测试：热稳定性用损耗率来描述。确定损失率

by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no
通过加速热降解试验，即将蛋白质在37°C下孵育48h，且不

obvious degradation and precipitation were observed. The loss rate is less than 5% within the
观察到明显的降解和降水。损失率小于5%以内

expiration date under appropriate storage condition.
在适当的储存条件下有效期。

[ SEQUENCE ] [ 序列 ]

[ IMPORTANT NOTE ] [ 重要提示 ]

The kit is designed for research use only, we will not be responsible for any issue if the kit was
该套件仅供研究使用，如果套件是

used in clinical diagnostic or any other procedures.
用于临床诊断或任何其他程序。

注意事项：该产品仅供科研实验使用，未经允许不得用于其它用途！以实际收货产品说明书为准，网站说明书仅供参考。